Other

Part:BBa_K277036:Design

Designed by: James DiCarlo   Group: iGEM09_Johns_Hopkins-BAG   (2009-10-21)


3L.3_23.B1.10


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 353
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 315
    Illegal PstI site found at 353
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 673
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 353
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 353
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 571


Design Notes

Constructed using overlap extension PCR protocol described in RFC38



Source

Synthetic Yeast

References